Paper Info
Title
An Aluminum Nano-Spike electroporation chip for low voltage delivery of molecules to cancer cells
Abstract
Micro electroporation (EP) devices with high efficiency and cell viability are useful for various biomedical applications, such as drug delivery and screening. However, these devices usually have low reliability due to undesirable electrochemical reactions. In this study, an Aluminum Nano-Spike EP (ANS-EP) chip was fabricated using nano-imprint lithography, electrochemical anodization and MEMS technology. The fabricated device was characterized using cervical cancer cell line (HeLa cells). The EP efficiency and cell viability as functions of electric pulse amplitude and duration were measured using digital fluorescent microscopy with Propidium Iodide (PI) dye. Numerical simulations of electric field strength indicated enhanced localized electric field at the tip due to high aspect ratio of nano-spikes. Systematic experiments revealed EP can be achieved with high efficiency and cell viability using ANS-EP chips without bubble generation at the critical applied voltage of 2.5 V, much smaller than the micro and conventional electroporators.
Year
DOI
Venue
2014
10.1109/NEMS.2014.6908779
Nano/Micro Engineered and Molecular Systems
Keywords
DocType
ISSN
aluminium,biomems,cancer,cellular transport,drug delivery systems,electrochemistry,electrophoresis,lab-on-a-chip,nanolithography,nanomedicine,numerical analysis,soft lithography,ans-ep chips,al,ep efficiency,hela cells,mems technology,propidium iodide dye,aluminum nano-spike electroporation chip,aspect ratio,biomedical applications,cell viability,cervical cancer cell line,conventional electroporators,digital fluorescent microscopy,drug delivery,drug screening,electric field strength,electric pulse amplitude,electric pulse duration,electrochemical anodization,electrochemical reactions,enhanced localized electric field,low voltage delivery,microelectroporation devices,microelectroporators,molecules,nanoimprint lithography,numerical simulations,voltage 2.5 v,aluminum nano-spike ep (ans-ep) chip,bubble generation,electroporation (ep) efficiency,ultra low voltage,aluminum,lab on a chip,fluorescence,electrodes,electric fields,fabrication
Conference
2474-3747
Citations 
PageRank 
References 
2
0.72
0
Authors
7
Name
Order
Citations
PageRank
k riaz120.72
s f leung220.72
shivendra tripathi320.72
gautam sethi420.72
h shagoshtasbi521.74
Zhiyong Fan621.74
Yi-Kuen Lee7711.16