Name
Title | ||
|---|---|---|
Viscosity effect on the Brownian relaxation based detection for immunoassay applications. |
Abstract | ||
|---|---|---|
Magnetic nanoparticles (MNPs) coated with Protein-G have been a model system to be used in different antibodies binding study. It is highly desirable to use a substrate-free biosensing system to detect antibodies binding in real-time. In this paper, we developed and applied a MNPs and search-coils integrated detection system, which is not only sensitive to the hydrodynamic volume of MNPs but also sensitive to the environment of MNPs, such as viscosity and temperature of the solution. We demonstrated that the viscosity effect influenced the amplitudes and phases of the 3rd (fH±2fL) and 5th (fH±4fL) harmonics for the mixed frequency testing scheme. The binding between antibodies and Protein-G on MNPs increased hydrodynamic volume of particles, as a result, it also changed the amplitudes and phases of harmonics, which are the object signals we need to analyze. We demonstrated that the viscosity of antibody solution is lower than that of MNP solution, and the antibody binding effect could be shielded by the viscosity effect to certain extent. |
Year | DOI | Venue |
|---|---|---|
2014 | 10.1109/EMBC.2014.6944197 | EMBC |
Keywords | Field | DocType |
biosensors,nanomedicine,nanoparticles,model system,hydrodynamic volume,nanosensors,antibody binding effect,solution temperature,viscosity effect,harmonic amplitudes,magnetic particles,mixed frequency testing scheme,proteins,antibody binding study,antibody solution viscosity,protein-g,molecular biophysics,antibody binding detection,substrate-free biosensing system,hydrodynamics,chemical sensors,magnetic nanoparticles,immunoassay applications,harmonic phases,search-coil integrated detection system,brownian motion,magnetic sensors,viscosity,brownian relaxation based detection,mnp solution | Nanotechnology,Computer science,Viscosity,Immunoassay,Brownian motion | Conference |
Volume | ISSN | Citations |
2014 | 1557-170X | 0 |
PageRank | References | Authors |
0.34 | 0 | 7 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Kai Wu | 1 | 21 | 9.25 |
| Lina Yu | 2 | 5 | 3.13 |
| Xiqian Zheng | 3 | 0 | 0.34 |
| Yi Wang | 4 | 0 | 0.34 |
| Yinglong Feng | 5 | 0 | 0.34 |
| Liang Tu | 6 | 0 | 0.34 |
| jianping wang | 7 | 40 | 5.87 |