Paper Info
Title
Protein-based analysis of alternative splicing in the human genome.
Abstract
Understanding the functional significance of alternative splicing and other mechanisms that generate RNA transcript diversity is an important challenge facing modern-day molecular biology. Using homology-based, protein sequence analysis methods, it should be possible to investigate how transcript diversity impacts protein structure and function. To test this, a data mining technique ("DiffHit") was developed to identify and catalog genes producing protein isoforms which exhibit distinct profiles of conserved protein motifs. We found that out of a test set of over 1,300 alternatively spliced genes with solved genomic structure, over 30% exhibited a differential profile of conserved InterPro and/or Blocks protein motifs across distinct isoforms. These results suggest that motif databases such as Blocks and InterPro are potentially useful tools for investigating how alternative transcript structure affects gene function.
Year
DOI
Venue
2002
10.1109/CSB.2002.1039335
Proceedings / IEEE Computer Society Bioinformatics Conference. IEEE Computer Society Bioinformatics Conference
Keywords
Field
DocType
human genome,motif databases,gene cataloguing,conserved blocks protein motifs,conserved protein motifs,protein isoforms,rna transcript diversity,genetics,gene identification,proteins,biology computing,molecular biology,protein-based analysis,differential profile,alternative splicing,protein function,data mining,protein structure,diffhit,conserved interpro protein motifs,homology-based protein sequence analysis,transcript structure
Gene,SNAP23,Protein sequencing,Biology,Protein engineering,Alternative splicing,Structural motif,RNA splicing,Bioinformatics,Genetics,InterPro
Conference
Volume
ISSN
ISBN
1
1555-3930
0-7695-1653-X
Citations 
PageRank 
References 
3
0.49
4
Authors
4
Name
Order
Citations
PageRank
Ann E. Loraine120625.20
Gregg A. Helt2767.07
Melissa S. Cline327143.65
Michael A. Siani-Rose49320.72