Paper Info
Title
A new equivalent circuit model for micro electroporation systems
Abstract
Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1~6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future.
Year
DOI
Venue
2011
10.1109/NEMS.2011.6017442
Nano/Micro Engineered and Molecular Systems
Keywords
Field
DocType
DNA,biomembranes,biomolecular electronics,biotechnology,electrodynamics,equivalent circuits,micromechanical devices,permeability,DNA molecules,HeLa cells,applied voltages,biotechnique,cell membrane,drugs,electric components,electrodynamic response,equivalent circuit model,exogenous biomolecules,intense electric pulses,membrane permeability,membrane resistance,micro EP system,micro electroporation systems,nanoscale electropores,transmembrane current responses,transmembrane voltage,voltage responses,Biomodeling,Electroporation,Equivalent circuit,MEMS,Transmembrane current
Analytical chemistry,Transfection,Voltage,Cell membrane,Membrane,Transmembrane protein,Permeability (electromagnetism),Materials science,Equivalent circuit,Electroporation
Conference
ISBN
Citations 
PageRank 
978-1-61284-775-7
0
0.34
References 
Authors
0
2
Name
Order
Citations
PageRank
h shagoshtasbi121.74
Yi-Kuen Lee2711.16