Name
Title | ||
|---|---|---|
Experimental validation of alternating transillumination for imaging intramural wave propagation. |
Abstract | ||
|---|---|---|
Current techniques to map intramural activation patterns in ex vivo cardiac tissue have limited spatial resolution. Here, we report on the experimental validation of a novel optical technique that was recently proposed to resolve the size and depth of intramural wave fronts using alternating transillumination (AT). AT was achieved by simultaneously mapping the epi- and endocardial surfaces with two synchronized CCD cameras and rapidly alternating LED illumination between both surfaces. Optical phantoms were made based on tissue optical properties measured using a hybrid optical spectrometer. Spherical fluorescent sources (Scarlet microspheres, Invitrogen, UK) of varying sizes were embedded at known depths in the phantoms. Coronary-perfused procine left ventricular slab preparations were stained with DI-4-ANBDQBS (n = 3) and paced at known intramural depths. In phantoms we were able to reliably estimate the depth of the center of fluorescent sources (9.6 ± 5.4% error), as well as their transmural extent (15.7 ± 11.5% error). In ventricular slabs we were able to localize the sites of origin of intramural excitation waves with a precision of ± 1.6 mm. Transmural conduction velocities were, for the first time, measured optically from the surfaces and found to be 21.0 ± 12.4 cm/s. In conclusion, alternating transillumination is a promising technique for reliable reconstruction of depth and expansion rate of intramural activation wave fronts in cardiac tissue. |
Year | DOI | Venue |
|---|---|---|
2011 | 10.1109/IEMBS.2011.6090482 | EMBC |
Keywords | Field | DocType |
spherical fluorescent sources,alternating transillumination,cardiology,lighting,biomedical optical imaging,rapidly alternating led illumination,spatial resolution,ventricular slab,ccd image sensors,cardiac tissue,synchronized ccd cameras,intramural activation patterns,scarlet microspheres,optical phantom,bio-optics,epicardial surfaces,intramural wave propagation imaging,transmural conduction velocity,biological tissues,endocardial,phantoms,heart conduction system,action potentials,fluorescence,optical imaging,ccd camera,wave propagation,surface wave,adaptive optics,conduction velocity | Biomedical engineering,Transillumination,Optical spectrometer,Wave propagation,Computer science,Voltage-Sensitive Dye Imaging,Optics,Excitation,Image resolution,Microsphere,Adaptive optics | Conference |
Volume | ISSN | ISBN |
2011 | 1557-170X | 978-1-4244-4122-8 |
Citations | PageRank | References |
1 | 0.48 | 0 |
Authors | ||
4 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| richard d walton | 1 | 3 | 3.04 |
| Christopher D Lawrence Xavier | 2 | 1 | 0.48 |
| Ilias Tachtsidis | 3 | 1 | 0.48 |
| Olivier Bernus | 4 | 7 | 5.37 |