Paper Info
Title
3D imaging for quantitative assessment of toxicity on vascular development in zebrafish.
Abstract
In this study, we describe the utility of the zebrafish model of in-vivo blood vessel formation as a tool for chemical risk assessment. Time-lapse confocal imaging of embryonic vasculature in the zebrafish is used in conjunction with digital image analysis to monitor and quantify the effect of toxins on vascular development. Non-rigid registration is used to capture changes in vascular morphology over time. Vascular formation in healthy normal and arsenic treated embryos was evaluated for differences in vascular structure using the algorithms developed. Although, the temporal progression of vascular development was similar, significant differences were observed in vessel structure between the toxin treated and healthy fish. This study revealed, for the first time, that vital vascular structures in fish maybe affected by exposure to arsenic. This technique allowed visualization of vascular abnormalities in embryos showing no external signs of malformations.
Year
DOI
Venue
2011
10.1109/IEMBS.2011.6091475
EMBC
Keywords
Field
DocType
temporal progression,vascular morphology,3d imaging,toxicity,zebrafish,biomedical optical imaging,blood vessels,time-lapse confocal imaging,in-vivo blood vessel formation,nonrigid registration,digital image analysis,image registration,toxicology,medical image processing,embryos,arsenic,risk assessment,biomedical imaging,spline,embryo
Toxicity,Biology,Embryo,Medical imaging,Zebrafish,Embryonic stem cell,Confocal,Quantitative assessment,Pathology,Blood vessel
Conference
Volume
ISSN
ISBN
2011
1557-170X
978-1-4244-4122-8
Citations 
PageRank 
References 
0
0.34
3
Authors
6
Name
Order
Citations
PageRank
Charu Hans100.34
Amol Shete200.34
Shishir K Shah350140.08
Catherine W McCollum411.07
Maria Bondesson511.75
Fatima A. Merchant612.76