Name
Title | ||
|---|---|---|
An experimental approach towards the development of an in vitro cortical-thalamic co-culture model. |
Abstract | ||
|---|---|---|
In this paper, we propose an experimental approach to develop an in vitro dissociated cortical-thalamic co-culture model using a dual compartment neurofluidic device. The device has two compartments separated by 10 μm wide and 3 μm high microchannels. The microchannels provide a physical isolation of neurons allowing only neurites to grow between the compartments. Long-term viable co-culture was maintained in the compartmented device, neurite growth through the microchannels was verified using immunofluorescence staining, and electrophysiological recordings from the co-culture system was investigated. Preliminary analysis of spontaneous activities from the co-culture shows a distinctively different firing pattern associated with cultures of individual cell types and further analysis is proposed for a deeper understanding of the dynamics involved in the network connectivity in such a co-culture system. |
Year | DOI | Venue |
|---|---|---|
2011 | 10.1109/IEMBS.2011.6090144 | EMBC |
Keywords | Field | DocType |
electrophysiology,in vitro,neuroscience,electrodes,microchannel | Network connectivity,Neuroscience,Biology,Artificial intelligence,Co-Culture,Neurite,Electrophysiology,Computer vision,Biophysics,In vitro,Compartment (ship),Cell type,Microchannel | Conference |
Volume | ISSN | ISBN |
2011 | 1557-170X | 978-1-4244-4122-8 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
6 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Kanagasabapathi Thirukumaran T | 1 | 0 | 0.34 |
| Massobrio Paolo | 2 | 0 | 0.34 |
| Tedesco Mariateresa | 3 | 0 | 0.34 |
| S Martinoia | 4 | 158 | 20.68 |
| Wadman Wytse J | 5 | 0 | 0.34 |
| Decré Michel M J | 6 | 0 | 0.34 |