Paper Info
Title
New automatic cell isolation system for flow cytometry: cell isolation unit and staining reagent kit.
Abstract
Flow cytometry is well-known cell analysis method and useful to gain quantitative information from cells in blood, however, it is not widely used for solid tissues in clinical settings. This is partly because it takes a long time to prepare samples and the operation can be complicated. To resolve these problems, we developed a new automatic cell isolation system which consists of cell isolation unit and staining reagent kit specialized for flow cytometry. With this new system, cell isolation can be done more rapidly and easily. By using this method, we could determine optimum condition to disintegrate porcine colon tissue and stain cells stably in 6 minutes. This result indicates that our method can provide analysis data within 10 minutes. We also evaluated our method in colorectal cancer patients, and the result was promising. All the data suggests that this method can support and facilitate rapid diagnosis.
Year
DOI
Venue
2012
10.1109/EMBC.2012.6346450
EMBC
Keywords
Field
DocType
solid tissues,cellular biophysics,rapid diagnosis,cell staining,cell analysis method,porcine colon tissue,staining reagent kit,automatic cell isolation system,sample preparation,cancer,colorectal cancer patients,cell isolation unit,patient diagnosis,flow cytometry,biological specimen preparation,blood cells
Biomedical engineering,Computer vision,Cellular biophysics,Flow cytometry,Stain,Computer science,Cell,Artificial intelligence,Staining,Molecular biology,Reagent
Conference
Volume
ISSN
ISBN
2012
1557-170X
978-1-4577-1787-1
Citations 
PageRank 
References 
0
0.34
0
Authors
12
Name
Order
Citations
PageRank
A Suzuki100.34
T Shioyama200.68
H Kubo300.34
Y Fukushima400.34
K Naemura500.34
N Hinata600.34
H Kanda701.01
S Yamamori800.34
S Takeda900.68
T Yamaguchi1000.34
Y Ishikawa1100.68
Y Kato1200.34