Paper Info
Title
Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish
Abstract
The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O2 plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.
Year
DOI
Venue
2012
10.1109/MHS.2012.6492441
Micro-NanoMechatronics and Human Science
Keywords
Field
DocType
biochemistry,biological tissues,biomedical materials,cellular biophysics,microfabrication,molecular biophysics,polymers,proteins,tissue engineering,O2 plasma treatment,chemical modification,extracted submandibular gland tissue,fibronectin coating,mesenchymal cells,microfabricated surface,micropatterned tissue culture dish,micropillar structure,physical modification,polydimethylsiloxane tissue culture substrate,surface affinity,tissue development
Biomedical engineering,Substrate (chemistry),Polydimethylsiloxane,Coating,Mesenchymal stem cell,In vitro,Tissue culture,Materials science,Fibronectin,Submandibular gland
Conference
ISSN
ISBN
Citations 
2474-378X
978-1-4673-4811-9
0
PageRank 
References 
Authors
0.34
0
2
Name
Order
Citations
PageRank
Takuya Matsumoto101.01
Seiji Aoyagi22514.63