Paper Info
Title
Modeling Study of the Light Stimulation of a Neuron Cell With Channelrhodopsin-2 Mutants
Abstract
Channelrhodopsin-2 (ChR2) has become a widely used tool for stimulating neurons with light. Nevertheless, the underlying dynamics of the ChR2-evoked spikes are still not yet fully understood. Here, we develop a model that describes the response of ChR2-expressing neurons to light stimuli and use the model to explore the light-to-spike process. We show that an optimal stimulation yield is achieved when the optical energies are delivered in short pulses. The model allows us to theoretically examine the effects of using various types of ChR2 mutants. We show that while increasing the lifetime and shuttering speed of ChR2 have limited effect, reducing the threshold irradiance by increased conductance will eliminate adaptation and allow constant dynamic range. The model and the conclusion presented in this study can help to interpret experimental results, design illumination protocols, and seek improvement strategies in the nascent optogenetic field.
Year
DOI
Venue
2011
10.1109/TBME.2011.2114883
Biomedical Engineering, IEEE Transactions
Keywords
Field
DocType
bioelectric phenomena,cellular biophysics,neurophysiology,photosynthesis,physiological models,proteins,channelrhodopsin-2 mutants,design illumination protocols,light stimulation,light-to-spike process,nascent optogenetic field,optical energies,optimal stimulation yield,photosynthesis,seek improvement strategies,shuttering speed,threshold irradiance,Biophysics,channelrhodopsin (ChR2),modeling,neurostimulation,optogenetics
Channelrhodopsin,Neuroscience,Cellular biophysics,Dynamic range,Optogenetics,Neurophysiology,Computer science,Stimulus (physiology),LIGHT STIMULATION,Neuron
Journal
Volume
Issue
ISSN
58
6
0018-9294
Citations 
PageRank 
References 
14
1.79
0
Authors
4
Name
Order
Citations
PageRank
Grossman, N.1141.79
Konstantin Nikolic2268.09
Christofer Toumazou326559.06
Patrick Degenaar45617.02