Title
Automated registration of live imaging stacks of Arabidopsis
Abstract
For actively developing tissues, a computational platform capable of automatically registering, segmenting and tracking cells is very critical to obtaining high-throughput and quantitative measurements of a range of cell behaviors, and can lead to a better understanding of the underlying dynamics of morphogenesis. In this work, we present an automated landmark-based registration method to register shoot apical meristem of Arabidopsis Thaliana images obtained through the Confocal Laser Scanning Microscopy technique. The proposed landmark-based registration method uses local graph-based approach to automatically find corresponding landmark pairs. The registration algorithm combined with an existing tracking method is tested on multiple datasets and it significantly improves the accuracy of cell lineages and division statistics.
Year
DOI
Venue
2013
10.1109/ISBI.2013.6556564
Biomedical Imaging
Keywords
Field
DocType
biological techniques,biology computing,cellular biophysics,image registration,optical microscopy,Arabidopsis thaliana image registration,automated landmark-based registration method,cell behavior,cell division statistics,cell lineage statistics,cell registration,cell segmentation,cell tracking,computational platform,confocal laser scanning microscopy technique,local graph-based approach,morphogenesis dynamics,shoot apical meristem,tissue development,tracking method,live imaging,registration,shoot apical meristem
Arabidopsis,Computer vision,Graph,Cellular biophysics,Stack (abstract data type),Pattern recognition,Computer science,Artificial intelligence,Landmark,Image registration,Live cell imaging,Confocal laser scanning microscopy
Conference
ISSN
ISBN
Citations 
1945-7928
978-1-4673-6456-0
4
PageRank 
References 
Authors
0.44
4
3
Name
Order
Citations
PageRank
Katya Mkrtchyan140.44
Anirban Chakraborty28510.00
Amit K. Roy Chowdhury3115373.96