Name
Title | ||
|---|---|---|
Fluorescence-based sensing of 2,4,6-trinitrotoluene (TNT) using a multi-channeled poly(methyl methacrylate) (PMMA) microimmunosensor. |
Abstract | ||
|---|---|---|
Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad- TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT. |
Year | DOI | Venue |
|---|---|---|
2010 | 10.3390/s100100876 | SENSORS |
Keywords | Field | DocType |
TNT,immunosensor,fluorescence,PMMA,microchannel,antibody | Embossing,Detection limit,Analytical chemistry,Siloxane,Trinitrotoluene,Fluorescence,Immunoassay,Methyl methacrylate,Engineering,Poly(methyl methacrylate) | Journal |
Volume | Issue | ISSN |
10 | 1 | 1424-8220 |
Citations | PageRank | References |
3 | 1.07 | 0 |
Authors | ||
6 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Paul T. Charles | 1 | 10 | 4.15 |
| Andre A. Adams | 2 | 5 | 1.95 |
| Peter B. Howell | 3 | 3 | 1.07 |
| Scott A. Trammell | 4 | 9 | 3.83 |
| Jeffrey R. Deschamps | 5 | 5 | 1.98 |
| Anne W. Kusterbeck | 6 | 5 | 1.95 |