Paper Info
Title
Influence Of Electrical Stimulation On 3d-Cultures Of Adipose Tissue Derived Progenitor Cells (Atdpcs) Behavior
Abstract
Tissue engineering has a fundamental role in regenerative medicine. Still today, the major motivation for cardiac regeneration is to design a platform that enables the complete tissue structure and physiological function regeneration of injured myocardium areas. Although tissue engineering approaches have been generally developed for two-dimensional (2D) culture systems, three-dimensional (3D) systems are being spotlighted as the means to mimic better in vivo cellular conditions. This manuscript examines the influence of electrical stimulation on 3D cultures of adipose tissue-derived progenitor cells (ATDPCs). ATDPCs cells were encapsulated into a self-assembling peptide nanoscaffold (RAD16-I) and continuously electro stimulated during 14-20 days with 2-ms pulses of 50mV/cm at a frequency of 1 Hz. Good cellular network formation and construct diameter reduction was observed in electro stimulated samples. Importantly, the process of electro stimulation does not disrupt cell viability or connectivity. As a future outlook, differentiation studies to cardiomyocytes-like cells will be performed analyzing gene profile and protein expression.
Year
DOI
Venue
2012
10.1109/EMBC.2012.6347278
2012 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)
Keywords
Field
DocType
cardiology,tissue engineering,self assembly
Biomedical engineering,Stem cell,Biology,Regenerative medicine,Cell biology,Electronic engineering,In vivo,Tissue engineering,Adipose tissue,Viability assay,Stimulation,Progenitor cell
Conference
Volume
ISSN
Citations 
2012
1557-170X
0
PageRank 
References 
Authors
0.34
0
6
Name
Order
Citations
PageRank
C Castells-Sala100.34
Benjamin Sanchez2177.49
L Recha-Sancho300.34
V Puig400.34
Bragos, R.531.61
C E Semino600.34