Name
Title | ||
|---|---|---|
Simultaneous characterization of instantaneous Young's modulus and specific membrane capacitance of single cells using a microfluidic system. |
Abstract | ||
|---|---|---|
This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young's modulus (E-instantaneous) and specific membrane capacitance (C-specific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (L-instantaneous) and transitional aspiration length (L-transitional), which was further translated to E-instantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine C-specific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing E-instantaneous of 2.96 +/- 0.40 kPa and C-specific membrane of 1.59 +/- 0.28 mu F/cm(2). As a platform for continuous and simultaneous characterization of cellular E-instantaneous and C-specific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties. |
Year | DOI | Venue |
|---|---|---|
2015 | 10.3390/s150202763 | SENSORS |
Keywords | Field | DocType |
microfluidics | Analytical chemistry,Membrane potential,Microfluidics,Young's modulus,Electrical impedance,Electronic engineering,Membrane,High-speed camera,Single-cell analysis,Engineering,Amplifier | Journal |
Volume | Issue | ISSN |
15 | 2 | 1424-8220 |
Citations | PageRank | References |
1 | 0.88 | 0 |
Authors | ||
10 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Yang Zhao | 1 | 1 | 0.88 |
| Deyong Chen | 2 | 10 | 3.03 |
| Yana Luo | 3 | 1 | 1.22 |
| Feng Chen | 4 | 1 | 0.88 |
| Xiaoting Zhao | 5 | 1 | 0.88 |
| Mei Jiang | 6 | 1 | 0.88 |
| Wentao Yue | 7 | 3 | 2.16 |
| Rong Long | 8 | 1 | 0.88 |
| Junbo Wang | 9 | 54 | 16.41 |
| Jian Chen | 10 | 23 | 15.42 |