Paper Info
Title
A choline oxidase amperometric bioassay for the detection of mustard agents based on screen-printed electrodes modified with Prussian Blue nanoparticles.
Abstract
In this work a novel bioassay for mustard agent detection was proposed. The bioassay is based on the capability of these compounds to inhibit the enzyme choline oxidase. The enzymatic activity, which is correlated to the mustard agents, was electrochemically monitored measuring the enzymatic product, hydrogen peroxide, by means of a screen-printed electrode modified with Prussian Blue nanoparticles. Prussian Blue nanoparticles are able to electrocatalyse the hydrogen peroxide concentration reduction at low applied potential (-50 mV vs. Ag/AgCl), thus allowing the detection of the mustard agents with no electrochemical interferences. The suitability of this novel bioassay was tested with the nitrogen mustard simulant bis(2-chloroethyl)amine and the sulfur mustard simulants 2-chloroethyl ethyl sulfide and 2-chloroethyl phenyl sulfide. The bioassay proposed in this work allowed the detection of mustard agent simulants with good sensitivity and fast response, which are excellent premises for the development of a miniaturised sensor well suited for an alarm system in case of terrorist attacks.
Year
DOI
Venue
2015
10.3390/s150204353
SENSORS
Keywords
Field
DocType
injury prevention,suicide prevention,biological assay,nanoparticles,ergonomics,electrodes,occupational safety,human factors
Sulfur mustard,Nanotechnology,Analytical chemistry,Nuclear chemistry,Amperometry,Chemistry,Nitrogen mustard,Choline oxidase,Hydrogen peroxide,Ferrocyanide,Mustard Agent,Prussian blue
Journal
Volume
Issue
ISSN
15
2.0
1424-8220
Citations 
PageRank 
References 
0
0.34
0
Authors
6
Name
Order
Citations
PageRank
Fabiana Arduini111.21
Viviana Scognamiglio200.68
Corrado Covaia300.34
Aziz Amine411.16
Danila Moscone511.21
Giuseppe Palleschi611.21