Name
Title | ||
|---|---|---|
Integration of a Miniature Quartz Crystal Microbalance with a Microfluidic Chip for Amyloid Beta-Aβ42 Quantitation. |
Abstract | ||
|---|---|---|
A miniature quartz crystal microbalance (mQCM) was integrated with a polydimethylsiloxane (PDMS) microfluidic device for on-chip determination of amyloid polypeptide-A(42). The integration techniques included photolithography and plasma coupling. A(42) antibody was immobilized on the mQCM surface using a cross-linker method, and the resonance frequency of mQCM shifted negatively due to antibody-antigen binding. A linear range from 0.1 mu M to 3.2 mu M was achieved. By using matrix elimination buffer, i.e., matrix phosphate buffer containing 500 mu g/mL dextran and 0.5% Tween 20, A(42) could be successfully detected in the presence of 75% human serum. Additionally, high temperature treatments at 150 degrees C provided a valid method to recover mQCM, and PDMS-mQCM microfluidic device could be reused to some extent. Since the detectable A(42) concentration could be as low as 0.1 mu M, which is close to cut-off value for Alzheimer patients, the PDMS-mQCM device could be applied in early Alzheimer's disease diagnosis. |
Year | DOI | Venue |
|---|---|---|
2015 | 10.3390/s151025746 | SENSORS |
Keywords | Field | DocType |
integration,miniature quartz crystal microbalance,polydimethylsiloxane,amyloid polypeptide-A(42) | Detection limit,Analytical chemistry,Polydimethylsiloxane,Quartz crystal microbalance,Microfluidics,Photolithography,Dextran,Linear range,Engineering,Chromatography,Quartz Crystal Microbalance Techniques | Journal |
Volume | Issue | ISSN |
15 | 10.0 | 1424-8220 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
6 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Wenyan Tao | 1 | 0 | 0.34 |
| Qingji Xie | 2 | 0 | 1.35 |
| Hairui Wang | 3 | 0 | 0.34 |
| Shanming Ke | 4 | 0 | 1.01 |
| Peng Lin | 5 | 0 | 1.35 |
| Xierong Zeng | 6 | 0 | 2.03 |