Paper Info
Title
Neuronal nuclei localization in 3D using level set and watershed segmentation from laser scanning microscopy images
Abstract
Abnormalities of the number and location of cells are hallmarks of both developmental and degenerative neurological diseases. However, standard stereological methods are impractical for assigning each cell's nucleus position within a large volume of brain tissue. We propose an automated approach for segmentation and localization of the brain cell nuclei in laser scanning microscopy (LSM) embryonic mouse brain images. The nuclei in these images are first segmented by using the level set (LS) and watershed methods in each optical plane. The segmentation results are further refined by application of information from adjacent optical planes and prior knowledge of nuclear shape. Segmentation is then followed with an algorithm for 31) localization of the centroid of nucleus (CN). Each volume of tissue is thus represented by a collection of centroids leading to an approximate 10,000-fold reduction in the data set size, as compared to the original image series. Our method has been tested on LSM images obtained from an embryonic mouse brain, and compared to the segmentation and CN localization performed by an expert. The average Euclidian distance between locations of CNs obtained using our method and those obtained by an expert is 1.58 +/- 1.24 mu m, a value well within the similar to 5 mu m average radius of each nucleus. We conclude that our approach accurately segments and localizes CNs within cell dense embryonic tissue.
Year
DOI
Venue
2008
10.1117/12.770849
PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)
Keywords
Field
DocType
level set segmentation,watershed segmentation,neurological diseases,neuronal nucleus segmentation,neuronal nucleus centroid localization
Computer vision,Nucleus,Pattern recognition,Biology,Segmentation,Euclidean distance,Image Series,Level set,Artificial intelligence,Microscopy,Embryonic Tissue,Centroid
Conference
Volume
ISSN
Citations 
6914
0277-786X
2
PageRank 
References 
Authors
0.37
5
6
Name
Order
Citations
PageRank
Yingxuan Zhu1132.85
eric c olson220.37
a subramanian320.71
David H. Feiglin4188.32
Pramod K. Varshney56689594.61
Andrzej Krol6298.49