Name
Abstract | ||
|---|---|---|
Despite the potential of stem cell-derived neural transplants for treating intractable neurological diseases, the global effects of a transplant's electrical activity on host circuitry have never been measured directly, preventing the systematic optimization of such therapies. Here, we overcome this problem by combining optogenetics, stem cell biology, and neuroimaging to directly map stem cell-driven neural circuit formation in vivo. We engineered human induced pluripotent stem cells (iPSCs) to express channelrhodopsin-2 and transplanted resulting neurons to striatum of rats. To non-invasively visualize the function of newly formed circuits, we performed high-field functional magnetic resonance imaging (fMRI) during selective stimulation of transplanted cells. fMRI successfully detected local and remote neural activity, enabling the global graft–host neural circuit function to be assessed. These results demonstrate the potential of a novel neuroimaging-based platform that can be used to identify how a graft's electrical activity influences the brain network in vivo. |
Year | DOI | Venue |
|---|---|---|
2015 | 10.1016/j.neuroimage.2015.03.079 | NeuroImage |
Keywords | Field | DocType |
iPSC,hESC | Induced pluripotent stem cell,Neuroscience,Optogenetics,Functional magnetic resonance imaging,Stem cell,Striatum,Psychology,In vivo,Neuroimaging,Biological neural network | Journal |
Volume | ISSN | Citations |
114 | 1053-8119 | 1 |
PageRank | References | Authors |
0.35 | 3 | 10 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Blake Byers | 1 | 1 | 0.35 |
| Hyun Joo Lee | 2 | 9 | 1.10 |
| Jia Liu | 3 | 10 | 1.49 |
| Andrew J. Weitz | 4 | 1 | 0.35 |
| Peter Lin | 5 | 9 | 1.10 |
| Pengbo Zhang | 6 | 42 | 7.69 |
| Aleksandr Shcheglovitov | 7 | 1 | 0.35 |
| Ricardo Dolmetsch | 8 | 1 | 0.35 |
| Renee Reijo Pera | 9 | 1 | 0.35 |
| Jin Hyung Lee | 10 | 48 | 7.33 |