Name
Title | ||
|---|---|---|
SPECT-imaging of activity-dependent changes in regional cerebral blood flow induced by electrical and optogenetic self-stimulation in mice. |
Abstract | ||
|---|---|---|
Electrical and optogenetic methods for brain stimulation are widely used in rodents for manipulating behavior and analyzing functional connectivities in neuronal circuits. High-resolution in vivo imaging of the global, brain-wide, activation patterns induced by these stimulations has remained challenging, in particular in awake behaving mice. We here mapped brain activation patterns in awake, intracranially self-stimulating mice using a novel protocol for single-photon emission computed tomography (SPECT) imaging of regional cerebral blood flow (rCBF). Mice were implanted with either electrodes for electrical stimulation of the medial forebrain bundle (mfb-microstim) or with optical fibers for blue-light stimulation of channelrhodopsin-2 expressing neurons in the ventral tegmental area (vta-optostim). After training for self-stimulation by current or light application, respectively, mice were implanted with jugular vein catheters and intravenously injected with the flow tracer 99m-technetium hexamethylpropyleneamine oxime (99mTc-HMPAO) during seven to ten minutes of intracranial self-stimulation or ongoing behavior without stimulation. The 99mTc-brain distributions were mapped in anesthetized animals after stimulation using multipinhole SPECT. Upon self-stimulation rCBF strongly increased at the electrode tip in mfb-microstim mice. In vta-optostim mice peak activations were found outside the stimulation site. Partly overlapping brain-wide networks of activations and deactivations were found in both groups. When testing all self-stimulating mice against all controls highly significant activations were found in the rostromedial nucleus accumbens shell. SPECT-imaging of rCBF using intravenous tracer-injection during ongoing behavior is a new tool for imaging regional brain activation patterns in awake behaving rodents providing higher spatial and temporal resolutions than 18F-2-fluoro-2-dexoyglucose positron emission tomography. |
Year | DOI | Venue |
|---|---|---|
2014 | 10.1016/j.neuroimage.2014.09.023 | NeuroImage |
Keywords | Field | DocType |
SPECT,PET,fMRI,mouse,reward,optogenetics | Neuroscience,Medial forebrain bundle,Nucleus accumbens,Brain stimulation,Psychology,Cerebral blood flow,Ventral tegmental area,Spect imaging,Stimulation,Preclinical imaging | Journal |
Volume | ISSN | Citations |
103 | 1053-8119 | 1 |
PageRank | References | Authors |
0.40 | 3 | 12 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Angela Kolodziej | 1 | 1 | 0.40 |
| Michael Lippert | 2 | 3 | 0.82 |
| Frank Angenstein | 3 | 30 | 5.56 |
| Jenni Neubert | 4 | 1 | 0.40 |
| Annette Pethe | 5 | 1 | 0.40 |
| Oliver S Grosser | 6 | 1 | 0.40 |
| Holger Amthauer | 7 | 2 | 1.15 |
| Ulrich H Schroeder | 8 | 1 | 0.40 |
| Klaus G Reymann | 9 | 1 | 0.40 |
| Henning Scheich | 10 | 147 | 29.04 |
| Frank W Ohl | 11 | 1 | 0.74 |
| Jürgen Goldschmidt | 12 | 5 | 1.72 |