Paper Info
Title
Development and Characterization of an Electroless Plated Silver/Cysteine Sensor Platform for the Electrochemical Determination of Aflatoxin B1.
Abstract
An electroless plated silver/cysteine sensor platform [Glass vertical bar silver vertical bar cysteine vertical bar aflatoxin B1 vertical bar horseradish peroxidase] for the Electro-chemical detection of aflatoxin B-1 was developed and characterized. This involved four major steps: (1) an electroless deposition of silver (plating) onto a glass slide, (2) immobilization of cysteine; (3) conjugation of aflatoxin B-1 to cysteine groups; and (4) blocking of free cysteine groups with horseradish peroxidase (HRP). The binding of cysteine to the silver was demonstrated by the disappearance of thiol (S-H) groups at 2500 cm(-1) using Fourier transmittance infrared spectra (FT-IR), while the subsequent steps in the assembly of sensor platform were monitored using both FT-IR and cyclic voltammetry, respectively. The sensor platform exhibited a broadened nonsymmetrical redox couple as indicated by cyclic voltammetry. The platform was further characterized for sensitivity and limit of detection. The indirect competitive immunoassay format, whereby free and immobilized aflatoxin B-1 on the sensor competed for the binding site of free anti-aflatoxin B-1 antibody, was used at various concentrations of aflatoxin B-1. The sensor generated differential staircase voltammogram that was inversely proportional to the concentration of aflatoxin B-1 and aflatoxin B-1 in the range of 0.06-1.1 ng/mL with a detection limit of 0.08 ng/mL could be detected.
Year
DOI
Venue
2016
10.1155/2016/3053019
JOURNAL OF SENSORS
DocType
Volume
ISSN
Journal
2016
1687-725X
Citations 
PageRank 
References 
0
0.34
0
Authors
5
Name
Order
Citations
PageRank
Alex Paul Wacoo100.34
Mathew Ocheng200.34
Deborah Wendiro300.34
Peter California Vuzi400.34
Joseph F. Hawumba500.34