Name
Title | ||
|---|---|---|
Nanogravimetric and Optical Characterizations of Thrombin Interaction with a Self-Assembled Thiolated Aptamer. |
Abstract | ||
|---|---|---|
Efficient biorecognition of thrombin (TB), a serine protease with crucial role in physiological and pathological blood coagulation, is a hot topic in medical diagnostics. In this work, we investigate the ability of synthetic thrombin aptamer (TBA), immobilized on a gold substrate, to bind thrombin by two different label-free techniques: the quartz crystal microbalance (QCM) and the spectroscopic ellipsometry (SE). By QCM characterization in the range from 20 to 110 nM, we demonstrate high specificity of TBA-TB interaction and determine affinity constant (K-d) of 17.7 +/- 0.3 nM, system sensitivity of 0.42 +/- 0.03 Hz nM(-1), and limit of detection (LOD) of 240 +/- 20 pM. The interaction between TBA and TB is also investigated by SE, an all-optical method, by quantifying the thickness increase of the TBA film assembled on gold substrate. AFM characterization of TBA and TB molecules deposited on flat silicon surface is also supplied. |
Year | DOI | Venue |
|---|---|---|
2016 | 10.1155/2016/3561863 | JOURNAL OF SENSORS |
Field | DocType | Volume |
Substrate (chemistry),Detection limit,Analytical chemistry,Aptamer,Quartz crystal microbalance,Molecule,Combinatorial chemistry,Thrombin,Chemistry,Electronic engineering,Ellipsometry,Silicon | Journal | 2016 |
ISSN | Citations | PageRank |
1687-725X | 0 | 0.34 |
References | Authors | |
1 | 9 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Jane Politi | 1 | 0 | 0.68 |
| Ilaria Rea | 2 | 0 | 1.69 |
| Fabrizia Nici | 3 | 0 | 0.34 |
| Principia Dardano | 4 | 0 | 1.01 |
| Monica Terracciano | 5 | 0 | 0.34 |
| Giorgia Oliviero | 6 | 0 | 0.34 |
| Nicola Borbone | 7 | 0 | 0.34 |
| Gennaro Piccialli | 8 | 0 | 0.34 |
| Luca De Stefano | 9 | 4 | 3.39 |