Name
Title | ||
|---|---|---|
Gene delivery to animal and plant cells using a nano-needle array-shaped photooxidative cell membrane perforator |
Abstract | ||
|---|---|---|
To avoid some cancer risks associated with the viral gene transfer process, the development of physical or chemical processes has received a great deal of attention in recent years. We found a unique cell membrane perforation method that applies photochemical oxidation to a limited cell membrane area, resulting in a high animal cell survival rate. We recently developed a nano-needle array cell membrane perforator that contains a photosensitizer for mass cell processing in a robotic system. In this research, we extended the gene transfer method to animal and plant cells. The targeted cells are human embryonic carcinoma cell line NT2/D1, onion epidermal cells, rice protoplasts and tobacco root calli. The cells were transformed with green fluorescent protein-expressing genes, and the transient expression of the genes was analyzed. Transient gene expression occurred and the adequate parameters for the expression were 400 gf/cm2 for 30 s for animal cells. The pressure and duration were in inverse proportions. The results in plant cells and protoplasts had similar parameters, but the plant cell walls required a relative high pressure, more than 50,000 gf/cm2. |
Year | DOI | Venue |
|---|---|---|
2015 | 10.1109/MHS.2015.7438326 | 2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS) |
Keywords | Field | DocType |
gene delivery,nanoneedle array-shaped photooxidative cell membrane perforator,cancer risk,viral gene transfer process,cell membrane perforation method,photochemical oxidation,cell membrane area,animal cell survival rate,photosensitizer,mass cell processing,robotic system,human embryonic carcinoma cell line,onion epidermal cell,rice protoplast,tobacco root calli,green fluorescent protein-expressing gene,transient gene expression,plant cell wall | Analytical chemistry,Gene delivery,Biology,Biochemistry,Plant cell,Gene expression,Embryonic stem cell,Cell biology,Cell membrane,Cell,Epidermis (botany),Cell wall | Conference |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
8 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Yukihiro Akiyama | 1 | 0 | 0.34 |
| Shunsuke Sakai | 2 | 0 | 0.34 |
| Suzuki, Y. | 3 | 0 | 0.68 |
| Shuhei Kashiwazaki | 4 | 0 | 0.34 |
| Shogo Unuma | 5 | 0 | 0.34 |
| Kei Innomata | 6 | 0 | 0.34 |
| Hiroetsu Wabiko | 7 | 0 | 0.34 |
| Takashi K Saito | 8 | 0 | 1.01 |