Paper Info
Title
High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection.
Abstract
We report an innovative fiber optic biosensor based on Long Period Gratings (LPGs) working in reflection configuration for real time monitoring of human Thyroglobulin (Tg), a protein marker of differentiated thyroid cancer. A standard LPG is first transformed in a practical probe working in reflection mode, and then it is coated with a single layer of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity and to provide, at the same time, the desired interfacial properties for a stable anti-Tg antibody. The functionalized reflection-type LPG biosensor clearly demonstrates the effectiveness and sensitivity of the developed biosensing platform, allowing the real time and label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels.
Year
DOI
Venue
2016
10.5220/0005846705610569
HEALTHINF
Field
DocType
Citations 
Biomedical engineering,Optical fiber,Data mining,Fiber,Computer science,Biotinylation,Long-period fiber grating,Analyte,Biosensor,Streptavidin,Thyroglobulin
Conference
0
PageRank 
References 
Authors
0.34
0
17
Name
Order
Citations
PageRank
Giuseppe Quero111.21
M. Consales221.39
Renato Severino300.34
Patrizio Vaiano400.34
Alessandra Boniello500.34
Annamaria Sandomenico600.34
Menotti Ruvo700.68
Anna Borriello800.68
Laura Diodato900.34
Simona Zuppolini1000.68
Michele Giordano1173.26
Immacolata Cristina Nettore1200.34
Annamaria Colao1300.34
Paolo Emidio Macchia1400.34
Flavio Santorelli1500.34
Antonello Cutolo1694.65
Andrea Cusano17105.85