Title
Towards automated neuron tracing via global and local 3D image analysis
Abstract
The reconstruction of the neural network is essential in computational neuroscience. Here, we present an automatic algorithm to trace single neuron projections based on two core algorithmic ideas: a global step segmenting all neuron bodies and their projections and a local growing phase that accommodates to the nonuniform illumination and to the noise of the sample. We tested our algorithm on two 3D stacks of two-photon images acquired from a human dysplastic brain sample. The results show that the traces produced are statistically equivalent to the ground truth, according to the Friedman and Li tests. Furthermore, we found that our algorithm outperforms other state-of-the-art methods.
Year
DOI
Venue
2016
10.1109/ISBI.2016.7493274
2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI)
Keywords
Field
DocType
Neuron tracing,Human brain,Two-photon microscopy,Neuron projections,BigNeuron
Computer vision,Computational neuroscience,Pattern recognition,Computer science,Ground truth,Artificial intelligence,Artificial neural network,Tracing,3d image
Conference
ISSN
Citations 
PageRank 
1945-7928
1
0.35
References 
Authors
2
7
Name
Order
Citations
PageRank
Ludovica Acciai120.70
irene costantini210.69
Francesco Saverio Pavone311.03
Valerio Conti410.35
Renzo Guerrini520.70
Paolo Soda640739.44
Giulio Iannello741446.75