Paper Info
Title
Microfluidic Platform With Integrated Thin-Film Optical Oxygen Sensors For Transient Hypoxia
Abstract
Physiologically, both chronic and cycling low oxygen level, or hypoxia, can alter tumour cell behaviour and reduce effectiveness of anticancer treatment. In order to create transiently hypoxic environments around tumour cells during drug screening, short diffusion distances between the control gases and the culture media are required in order to reproduce the timescales of a few cycles per hour that have been observed in animal studies. We use a gas-permeable three-layer microfluidic device to achieve spatial and temporal oxygen control with equilibration time less than 10 minutes. We integrate thin optical oxygen sensing films composed of polystyrene with an embedded luminescent oxygen-sensitive dye in order to monitor oxygen levels within the system. We show that the microfluidic oxygen control afforded by this design is sufficient to achieve oxygen concentrations below the sensor limit of detection of 0.08% in the fluidic cell culture environment. This platform allows us to generate complex, time-varying oxygen profiles. The sensors are biocompatible and engineered tumour spheroids cultured in this platform show good viability and cell proliferation over the course of several days with in situ oxygen monitoring.
Year
Venue
Keywords
2016
2016 14TH IEEE INTERNATIONAL NEW CIRCUITS AND SYSTEMS CONFERENCE (NEWCAS)
optical sensor, hypoxia, microfluidic
Field
DocType
ISSN
Fluidics,Biomedical engineering,Nanotechnology,Polystyrene,Spheroid,Computer science,Oxygen,Microfluidics,Oxygen sensor,Oxygen saturation (medicine),Electronic engineering,Thin film
Conference
2472-467X
Citations 
PageRank 
References 
0
0.34
1
Authors
4
Name
Order
Citations
PageRank
Samantha M Grist193.19
Jonathan C. Schmok200.34
Andrea Diaz Gaxiola300.34
Karen C Cheung428520.22