Name
Title | ||
|---|---|---|
Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen. |
Abstract | ||
|---|---|---|
In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342-137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 +/- 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs. |
Year | DOI | Venue |
|---|---|---|
2016 | 10.3390/s16122154 | SENSORS |
Keywords | Field | DocType |
gold,nanoparticle,hepatitis B surface antigen,lateral flow assay,conjugation | Hepatitis B Surface Antigens,Analytical chemistry,Antigen,Colloidal gold,Particle size,Colorimetry,Engineering,Biosensor,Conjugated system,Nanoparticle | Journal |
Volume | Issue | ISSN |
16 | 12.0 | 1424-8220 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
11 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Dong Seok Kim | 1 | 11 | 2.27 |
| Yong Tae Kim | 2 | 22 | 8.62 |
| Seok Bok Hong | 3 | 0 | 0.34 |
| Jinwoon Kim | 4 | 0 | 0.34 |
| Nam Su Heo | 5 | 0 | 1.01 |
| Moon-Keun Lee | 6 | 0 | 0.34 |
| Seok Jae Lee | 7 | 3 | 2.84 |
| Byeong-Il Kim | 8 | 0 | 0.34 |
| In Soo Kim | 9 | 0 | 0.34 |
| Yun Suk Huh | 10 | 0 | 0.68 |
| Bong Gill Choi | 11 | 0 | 0.34 |