Name
Abstract | ||
|---|---|---|
Existing intracellular electrodes possess the disadvantages of causing cell death via mechanical stress during the tip insertion through the cell membrane, and integration difficulty because the electrode structure is based on glass capillaries. Recent research on nano-scale metal microelectrodes for intracellular recording reports that the microelectrode tip scarcely penetrates the cell membrane because of the flexibility and fluidity of the membrane, and may induce cell destruction if the penetration process forces too much stress into the cell. This study reports the development of an electrode possessing a photochemical cell membrane perforation function that introduces focused oxidative damage to the cell membrane with a light-activated photosensitizer. The electrode is based on a sub-micrometer-scale platinum wire, which cannot alone penetrate the membrane. The electrode wire is coated with the photosensitizer hematoporphyrin contained in polydimethylsiloxane. The coating layer works as an insulator, and the layer on the tip is removed by focused ion beam etching. The electrode is applied to the rat pheochromocytoma PC12 cell line and successfully obtains electrical signals from the targeted cell following light irradiation of the electrode. The developed electrode is single-wire based, but this fabrication method can be easily applied to integrated electrodes. |
Year | DOI | Venue |
|---|---|---|
2016 | 10.1109/MHS.2016.7824199 | 2016 International Symposium on Micro-NanoMechatronics and Human Science (MHS) |
Keywords | Field | DocType |
sub-micrometer-scale wire-based intracellular metal microelectrode,cell death,mechanical stress,electrode structure,glass capillaries,nanoscale metal microelectrodes,intracellular recording,membrane fluidity,photochemical cell membrane perforation function,oxidative damage,light-activated photosensitizer,sub-micrometer-scale platinum wire,photosensitizer hematoporphyrin,polydimethylsiloxane,coating layer,ion beam etching,rat pheochromocytoma PC12 cell line,electrical signals,light irradiation,integrated electrodes,Pt | Nanotechnology,Analytical chemistry,Polydimethylsiloxane,Coating,Intracellular,Cell membrane,Membrane,Focused ion beam,Materials science,Microelectrode,Electrode | Conference |
ISBN | Citations | PageRank |
978-1-5090-2786-6 | 0 | 0.34 |
References | Authors | |
0 | 3 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Naoto Ito | 1 | 0 | 0.68 |
| Naoto Konno | 2 | 0 | 0.34 |
| Takashi K Saito | 3 | 0 | 1.01 |