Paper Info
Title
An Improved Label-Free Indirect Competitive SPR Immunosensor and Its Comparison with Conventional ELISA for Ractopamine Detection in Swine Urine.
Abstract
Ractopamine (RCT) is banned for use in animals in many countries, and it is urgent to develop efficient methods for specific and sensitive RCT detection. A label-free indirect competitive surface plasmon resonance (SPR) immunosensor was first developed with a primary antibody herein and then improved by a secondary antibody for the detection of RCT residue in swine urine. Meanwhile, a pre-incubation process of RCT and the primary antibody was performed to further improve the sensitivity. With all the key parameters optimized, the improved immunosenor can attain a linear range of 0.3-32 ng/mL and a limit of detection (LOD) of 0.09 ng/mL for RCT detection with high specificity. Furthermore, the improved label-free SPR immunosenor was compared thoroughly with a conventional enzyme-linked immunosorbent assay (ELISA). The SPR immunosensor showed advantages over the ELISA in terms of LOD, reagent consumption, analysis time, experiment automation, and so on. The SPR immunosensor can be used as potential method for real-time monitoring and screening of RCT residue in swine urine or other samples. In addition, the design using antibody pairs for biosensor development can be further referred to for other small molecule detection.
Year
DOI
Venue
2017
10.3390/s17030604
SENSORS
Keywords
Field
DocType
SPR immunosensor,ractopamine,swine urine,ELISA
Detection limit,Urine,Analytical chemistry,Surface plasmon resonance,Primary and secondary antibodies,Ractopamine,Chemistry,Linear range,Biosensor,Chromatography
Journal
Volume
Issue
Citations 
17
3.0
1
PageRank 
References 
Authors
0.43
0
6
Name
Order
Citations
PageRank
Sai Wang110.43
Shuai Zhao210.43
Xiao Wei36011.63
Shan Zhang4133.33
Jiahui Liu510.43
Yiyang Dong610.43