Paper Info
Title
Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection.
Abstract
A novel assembled biosensor was prepared for detecting 16S rRNA, a small-size persistent specific for Actinobacteria. The mechanism of the porous silicon (PS) photonic crystal biosensor is based on the fluorescence resonance energy transfer (FRET) between quantum dots (QDs) and gold nanoparticles (AuNPs) through DNA hybridization, where QDs act as an emission donor and AuNPs serve as a fluorescence quencher. Results showed that the photoluminescence (PL) intensity of PS photonic crystal was drastically increased when the QDs-conjugated probe DNA was adhered to the PS layer by surface modification using a standard cross-link chemistry method. The PL intensity of QDs was decreased when the addition of AuNPs-conjugated complementary 16S rRNA was dropped onto QDs-conjugated PS. Based on the analysis of different target DNA concentration, it was found that the decrease of the PL intensity showed a good linear relationship with complementary DNA concentration in a range from 0.25 to 10 mu M, and the detection limit was 328.7 nM. Such an optical FRET biosensor functions on PS-based photonic crystal for DNA detection that differs from the traditional FRET, which is used only in liquid. This method will benefit the development of a new optical FRET label-free biosensor on Si substrate and has great potential in biochips based on integrated optical devices.
Year
DOI
Venue
2017
10.3390/s17051078
SENSORS
Keywords
Field
DocType
FRET,DNA biosensor,porous silicon,photonic crystal
Nanotechnology,Analytical chemistry,Photonic crystal,Porous silicon,Colloidal gold,Förster resonance energy transfer,Engineering,Biosensor,Quenching (fluorescence),Quantum dot,Photoluminescence
Journal
Volume
Issue
ISSN
17
5.0
1424-8220
Citations 
PageRank 
References 
3
0.99
0
Authors
3
Name
Order
Citations
PageRank
Hongyan Zhang153.74
Jie Lv241.76
Zhenhong Jia32915.13