Title
Cell segmentation in 3D confocal images using supervoxel merge-forests with CNN-based hypothesis selection
Abstract
Automated segmentation approaches are crucial to quantitatively analyze large-scale 3D microscopy images. Particularly in deep tissue regions, automatic methods still fail to provide error-free segmentations. To improve the segmentation quality throughout imaged samples, we present a new supervoxel-based 3D segmentation approach that outperforms current methods and reduces the manual correction effort. The algorithm consists of gentle preprocessing and a conservative super-voxel generation method followed by supervoxel agglomeration based on local signal properties and a postprocessing step to fix under-segmentation errors using a Convolutional Neural Network. We validate the functionality of the algorithm on manually labeled 3D confocal images of the plant Arabidopsis thaliana and compare the results to a state-of-the-art meristem segmentation algorithm.
Year
DOI
Venue
2018
10.1109/ISBI.2018.8363598
2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018)
Keywords
DocType
Volume
Cell Segmentation,Convolutional Neural Networks,Developmental Biology,Arabidopsis,Meristem
Conference
abs/1710.06608
ISSN
ISBN
Citations 
1945-7928
978-1-5386-3637-4
1
PageRank 
References 
Authors
0.36
6
7
Name
Order
Citations
PageRank
Johannes Stegmaier1369.21
Thiago Vallin Spina210.36
Alexandre X. Falcão31877132.30
Andreas Bartschat4143.48
Ralf Mikut518835.34
Elliot M Meyerowitz65511.09
Alexandre Cunha721.04