Paper Info
Title
Amyloid Beta Detection by Faradaic Electrochemical Impedance Spectroscopy Using Interdigitated Microelectrodes.
Abstract
Faradaic electrochemical impedance spectroscopy (f-EIS) in the presence of redox reagent, e.g., [Fe(CN)(6)](3/4), is widely used in biosensors owing to its high sensitivity. However, in sensors detecting amyloid beta (A beta), the redox reagent can cause the aggregation of A beta, which is a disturbance factor in accurate detection. Here, we propose an interdigitated microelectrode (IME) based f-EIS technique that can alleviate the aggregation of A beta and achieve high sensitivity by buffer control. The proposed method was verified by analyzing three different EIS-based sensors: non-faradaic EIS (nf-EIS), f-EIS, and the proposed f-EIS with buffer control. We analyzed the equivalent circuits of nf-EIS and f-EIS sensors. The dominant factors of sensitivity were analyzed, and the impedance change rates via A beta reaction was compared. We measured the sensitivity of the IME sensors based on nf-EIS, f-EIS, and the proposed f-EIS. The results demonstrate that the proposed EIS-based IME sensor can detect A beta with a sensitivity of 7.40-fold and 10.93-fold higher than the nf-EIS and the f-EIS sensors, respectively.
Year
DOI
Venue
2018
10.3390/s18020426
SENSORS
Keywords
Field
DocType
amyloid beta,redox reagent,[Fe(CN)(6)](3-/4-),faradaic electrochemical impedance spectroscopy,biosensor,high sensitivity
Analytical chemistry,Electrical impedance,Dielectric spectroscopy,Engineering,Biosensor,Amyloid beta,Equivalent circuit,Reagent,Microelectrode,Redox
Journal
Volume
Issue
ISSN
18
2.0
1424-8220
Citations 
PageRank 
References 
1
0.36
0
Authors
7
Name
Order
Citations
PageRank
j s park121.73
Hye-Jin Kim2212.02
Jihoon Lee335.62
Jung Ho Park4142.92
Jinsik Kim511.04
kyo hwang642.51
Byung-chul Lee741.86