Name
Title | ||
|---|---|---|
Label-Free and Redox Markers-Based Electrochemical Aptasensors for Aflatoxin M1 Detection. |
Abstract | ||
|---|---|---|
We performed a comparative analysis of the sensitivity of aptamer-based biosensors for detection mycotoxin aflatoxin M-1 (AFM1) depending on the method of immobilization of DNA aptamers and method of the detection. Label-free electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) for ferrocene labeled neutravidin layers were used for this purpose. Amino-modified DNA aptamers have been immobilized at the surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) or biotin-modified aptamers were immobilized at the neutravidin layer chemisorbed at gold surface. In the first case the limit of detection (LOD) has been determined as 8.47 ng/L. In the second approach the LOD was similar 8.62 ng/L, which is below of allowable limits of AFM1 in milk and milk products. The aptasensors were validated in a spiked milk samples with good recovery better than 78%. Comparative analysis of the sensitivity of immuno-and aptasensors was also performed and showed comparable sensitivity. |
Year | DOI | Venue |
|---|---|---|
2018 | 10.3390/s18124218 | SENSORS |
Keywords | Field | DocType |
biosensors,aflatoxin M1,aptasensors,electrochemical impedance spectroscopy,differential pulse voltammetry | Nanotechnology,Analytical chemistry,Aflatoxin,Engineering,Electrochemistry,Redox | Journal |
Volume | Issue | ISSN |
18 | 12.0 | 1424-8220 |
Citations | PageRank | References |
0 | 0.34 | 2 |
Authors | ||
3 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Stefanos Karapetis | 1 | 0 | 0.34 |
| Dimitrios Nikolelis | 2 | 0 | 0.34 |
| Tibor Hianik | 3 | 0 | 3.04 |