Paper Info
Title
A Novel Mass-Producible Capacitive Sensor with Fully Symmetric 3D Structure and Microfluidics for Cells Detection.
Abstract
Affinity biosensors of interdigitated electrodes have been widely used in cell detection. This research presents a mass-producible and disposable three-dimensional (3D) structure capacitive sensor based on the integrated circuit package lead frames for cell concentration detection. The fully symmetric 3D interdigital electrode structure makes the sensor more homogeneous and sensitive. (3-Aminopropyl) triethoxysilane (APTES) and glutaraldehyde are immobilized onto gold-plated electrodes. By overlaying the microfluidic channels on top, the volume of the solution is kept constant to obtain repeatable measured capacitance values. Moreover, using the upgraded reading and writing functions and circular measurement of the E4980A Data Transfer Program, an automatic multigroup test system is developed. It is shown that the cell concentration and capacitance are inversely correlated, and the cell concentration range of 10(3)-10(6) CFU.mL(-1) is achieved. In addition, the rate of capacitance change matches that of state-of-the-art biosensors reported. A program is developed to find the optimal voltage and frequency for linear fitting between the capacitance change and cell concentration. Future work will employ machine learning-based data analysis to drug resistance sensitivity test of cell lines and cell survival status.
Year
DOI
Venue
2019
10.3390/s19020325
SENSORS
Keywords
Field
DocType
capacitance sensor,biosensor,biochip,affinity sensor,microfluidics,cell detection,multidimensional data
Biochip,Capacitance,Voltage,Microfluidics,Capacitive sensing,Electronic engineering,Engineering,Biosensor,Optoelectronics,Integrated circuit,Electrode
Journal
Volume
Issue
ISSN
19
2.0
1424-8220
Citations 
PageRank 
References 
0
0.34
0
Authors
6
Name
Order
Citations
PageRank
Zhaorui Zuo100.34
Kun Wang215045.41
Libin Gao300.34
Vincent Ho400.34
Hongju Mao500.34
Dahong Qian6277.78