Title | ||
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Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio |
Abstract | ||
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This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60µm) ratio higher than 60%, suitable for in-vitro fertilization (IVF). |
Year | DOI | Venue |
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2016 | 10.1109/NEMS.2016.7758215 | 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) |
Keywords | DocType | ISSN |
Sperm,Chip,PDMS,CASA | Conference | 2474-3747 |
ISBN | Citations | PageRank |
978-1-5090-1948-9 | 0 | 0.34 |
References | Authors | |
0 | 9 |
Name | Order | Citations | PageRank |
---|---|---|---|
Yueh Jen Chen | 1 | 0 | 0.68 |
Ren-Guei Wu | 2 | 10 | 3.48 |
pengchun chen | 3 | 0 | 0.68 |
yunan lin | 4 | 0 | 0.68 |
Huei Wen Hsiu | 5 | 0 | 0.34 |
Ching Chu Yen | 6 | 0 | 0.34 |
Chia-Woei Wang | 7 | 0 | 0.34 |
Li-Chern Pan | 8 | 0 | 0.34 |
Fan-Gang Tseng | 9 | 16 | 11.92 |