Title
Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio
Abstract
This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60µm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).
Year
DOI
Venue
2016
10.1109/NEMS.2016.7758215
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
Keywords
DocType
ISSN
Sperm,Chip,PDMS,CASA
Conference
2474-3747
ISBN
Citations 
PageRank 
978-1-5090-1948-9
0
0.34
References 
Authors
0
9
Name
Order
Citations
PageRank
Yueh Jen Chen100.68
Ren-Guei Wu2103.48
pengchun chen300.68
yunan lin400.68
Huei Wen Hsiu500.34
Ching Chu Yen600.34
Chia-Woei Wang700.34
Li-Chern Pan800.34
Fan-Gang Tseng91611.92