Paper Info
Title
Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens.
Abstract
A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 10(2)-10(3) CFUmL(-1) in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.
Year
DOI
Venue
2017
10.3390/s17030442
SENSORS
Keywords
Field
DocType
food-borne pathogens,fluorescence,automated instrument system,on-site detection,quantum dots,LabVIEW
Detection limit,Reproducibility,Analytical chemistry,Fluorescence,Diode,Spectrometer,Electronic engineering,Software,Engineering,Quantum dot
Journal
Volume
Issue
Citations 
17
3.0
0
PageRank 
References 
Authors
0.34
1
7
Name
Order
Citations
PageRank
Zhan Lu100.34
Jianyi Zhang200.34
Lizhou Xu300.34
Yanbin Li472.85
Siyu Chen5295.96
Zunzhong Ye691.72
Jianping Wang700.68