Name
Title | ||
|---|---|---|
Hand-held Colorimetry Sensor Platform for Determining Salivary α-Amylase Activity and Its Applications for Stress Assessment. |
Abstract | ||
|---|---|---|
This study develops a hand-held stress assessment meter with a chemically colorimetric strip for determining salivary -amylase activity, using a 3,5 dinitrosalicylic acid (DNS) assay to quantify the reducing sugar released from soluble starch via -amylase hydrolysis. The colorimetric reaction is produced by heating the strip with a mini polyester heater plate at boiling temperature to form a brick red colored product, which measured at 525 nm wavelength. This investigation describes in detail the design, construction, and performance evaluation of a hand-held -amylase activity colorimeter with a light emitted diode (LED) and photo-detector with built-in filters. The dimensions and mass of the proposed prototype are only 120 x 60 x 60 mm(3) and 200 g, respectively. This prototype has an excellent correlation coefficient (>0.995), comparable with a commercial ultraviolet-visible spectroscope, and has a measurable -amylase activity range of 0.1-1.0 U mL(-1). The hand-held device can measure the salivary -amylase activity with only 5 L of saliva within 12 min of testing. This sensor platform effectively demonstrates that the level of salivary -amylase activity increases more significantly than serum cortisol, the other physiological stressor biomarker, under physiologically stressful exercise conditions. Thus, this work demonstrates that the hand-held -amylase activity meter is an easy to use and cost-effective stress assessment tool for psychoneuroendocrinology research. |
Year | DOI | Venue |
|---|---|---|
2019 | 10.3390/s19071571 | SENSORS |
Keywords | Field | DocType |
hand-held optical sensing meter,stress assessment,salivary -amylase,colorimetry | Correlation coefficient,Analytical chemistry,Hydrolysis,Colorimeter,Reducing sugar,Amylase,Colorimetry,Engineering,Starch,Saliva | Journal |
Volume | Issue | ISSN |
19 | 7.0 | 1424-8220 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
4 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Hsien-Yi Hsiao | 1 | 0 | 0.34 |
| Richie L C Chen | 2 | 0 | 0.34 |
| Chih-Chi Chou | 3 | 0 | 0.34 |
| Tzong-Jih Cheng | 4 | 0 | 0.34 |