Name
Title | ||
|---|---|---|
Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase. |
Abstract | ||
|---|---|---|
Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM. |
Year | DOI | Venue |
|---|---|---|
2019 | 10.3390/s19071677 | SENSORS |
Keywords | Field | DocType |
protease biosensor,collagenase,poly(ethylene glycol) norbornene,QCM,hydrogel degradation,peptide cross-links,click chemistry | Detection limit,Analytical chemistry,Quartz crystal microbalance,Ethylene glycol,Peptide,Collagenase,Tissue engineering,Chromatography,Engineering,Biosensor,Drug delivery | Journal |
Volume | Issue | ISSN |
19 | 7.0 | 1424-8220 |
Citations | PageRank | References |
0 | 0.34 | 0 |
Authors | ||
8 | ||
Name | Order | Citations | PageRank |
|---|---|---|---|
| Norlaily Ahmad | 1 | 0 | 0.34 |
| Burcu Colak | 2 | 0 | 0.34 |
| De-Wen Zhang | 3 | 0 | 0.34 |
| Martin John Gibbs | 4 | 0 | 0.34 |
| Michael Watkinson | 5 | 0 | 0.34 |
| C Remzi Becer | 6 | 0 | 0.34 |
| Julien E Gautrot | 7 | 0 | 0.34 |
| Steffi Krause | 8 | 0 | 0.68 |