Title
Assessing microscope image focus quality with deep learning.
Abstract
Our deep neural network enables classification of out-of-focus microscope images with both higher accuracy and greater precision than previous approaches via interpretable patch-level focus and certainty predictions. The use of synthetically defocused images precludes the need for a manually annotated training dataset. The model also generalizes to different image and cell types. The framework for model training and image prediction is available as a free software library and the pre-trained model is available for immediate use in Fiji (ImageJ) and CellProfiler.
Year
DOI
Venue
2018
10.1186/s12859-018-2087-4
BMC Bioinformatics
Keywords
Field
DocType
CellProfiler,Deep learning,Defocus,Focus,Image analysis,Image quality,ImageJ,Machine learning,Open-source
Biology,Autofocus,Pattern recognition,Medical imaging,Image quality,Software,Pixel,Artificial intelligence,Deep learning,Bioinformatics,Artificial neural network,Hoechst stain
Journal
Volume
Issue
ISSN
19
1
1471-2105
Citations 
PageRank 
References 
3
0.44
4
Authors
13