Paper Info
Title
Uptake of L-maurocalcine in DAOY cells and bio-distribution in mice by SPECT/CT imaging.
Abstract
Background: Cell permeable peptides (CPP) are a new class of carrier molecule to deliver biomolecules, radionucleotide and drugs that is gaining momentum. CPP are capable of entering into the cells by breaking the resistance of the membrane barrier and thus can be used universally in many cell types, which renders it an efficient carrier for both in-vitro and in-vivo use. Methods: L-Maurocalcine (L-MCa), a peptide derived from scorpion venom was radiolabeled with 125 I using the lactoperoxidase method. We achieved a specific activity of 45Mbq/nmole. In vitro studies with I-125-L-MCa in DAOY cells (human medulloblastoma) were studied in order to analyze the uptake of the peptide. I-125-L-MCa was injected intravenously in mice through tail vein and bio-distribution was studied using single photon emission tomography/computed tomography (SPECT/CT). Results: The cellular uptake of the I-125-L-MCa in DAOY cells was time and dose dependent suggesting that the radiolabeled peptide retains the biological property after radiolabeling. We have observed no loss of cell viability upon uptake of I-125-L-MCa, favoring that this peptide has potential for use in in vivo studies. The distribution of the I-125-L-MCa in mice revealed its uptake in the liver, kidney and stomach. Interestingly the I-125-L-MCa was cleared from the circulation 24h post injection, thus providing another advantage for its use in in vivo studies. Conclusions: In the present study we have shown the uptake of I-125-L-MCa in DAOY cells. Further, the I-125-L-MCa when injected in mice localized to the liver, kidney and stomach as revealed by SPECT/CT. Cells labeled with I-125-L-MCa can possibly be tracked to their target site.
Year
DOI
Venue
2018
10.1117/12.2293973
Proceedings of SPIE
Keywords
DocType
Volume
DAOY Cells,L-MCa,CPP,Radio-iodination,SPECT/CT
Conference
10578
ISSN
Citations 
PageRank 
0277-786X
0
0.34
References 
Authors
0
2
Name
Order
Citations
PageRank
Poonam Sarkar100.68
M. Waleed Gaber201.01