Name
Title | ||
|---|---|---|
Selectively Capturing Monocyte from Whole Blood on Microfluidic Biochip for Sepsis Diagnosis |
Abstract | ||
|---|---|---|
Sepsis, a clinical syndrome caused by infection, has been frequently observed in the patients who are young, old, and with a weakened immune system. The low expression of HLA-DR (Human leukocyte antigen) on the surface of leukocyte is believed to reliably examine the sepsis patient's immune system. We report a microfluidic biochip to quickly separate leukocytes from minute whole blood sample by using deterministic lateral displacement and deposit leukocytes into antibody-coating micro-holes matrix. The monocytes are selectively captured by antibody while other leukocytes are dropped by flipping the device. Currently, our biochip can capture surface-modified THP-1 which are utilized to mimic monocytes and can selectively capture THP-1 from mixed sample of THP-1 and beads. |
Year | DOI | Venue |
|---|---|---|
2018 | 10.1109/NEMS.2018.8556870 | 2018 IEEE 13th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) |
Keywords | DocType | ISSN |
surface-modified THP-1,monocytes,capturing monocyte,microfluidic biochip,sepsis diagnosis,sepsis patient,deposit leukocytes,human leukocyte antigen,antibody-coating microhole matrix | Conference | 2474-3747 |
ISBN | Citations | PageRank |
978-1-5386-5274-9 | 0 | 0.34 |
References | Authors | |
0 | 4 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Chung-Yu Chou | 1 | 0 | 0.34 |
| Yen-Ta Lu | 2 | 0 | 0.68 |
| Chia-Ming Chang | 3 | 559 | 34.44 |
| Cheng-Hsien Liu | 4 | 0 | 3.72 |