Paper Info
Title
Label-Free Detection of E. coli O157:H7 DNA Using Light-Addressable Potentiometric Sensors with Highly Oriented ZnO Nanorod Arrays.
Abstract
The detection of bacterial deoxyribonucleic acid (DNA) is of great significance in the quality control of food and water. In this study, a light-addressable potentiometric sensor (LAPS) deposited with highly oriented ZnO nanorod arrays (NRAs) was used for the label-free detection of single-stranded bacterial DNA (ssDNA). A functional, sensitive surface for the detection of Escherichia coli (E. coli) O157:H7 DNA was prepared by the covalent immobilization of the specific probe single-stranded DNA (ssDNA) on the LAPS surface. The functional surface was exposed to solutions containing the target E. coli ssDNA molecules, which allowed for the hybridization of the target ssDNA with the probe ssDNA. The surface charge changes induced by the hybridization of the probe ssDNA with the target E. coli ssDNA were monitored using LAPS measurements in a label-free manner. The results indicate that distinct signal changes can be registered and recorded to detect the target E. coli ssDNA. The lower detection limit of the target ssDNA corresponded to 1.0 x 10(2) colony forming units (CFUs)/mL of E. coli O157:H7 cells. All the results demonstrate that this DNA biosensor, based on the electrostatic detection of ssDNA, provides a novel approach for the sensitive and effective detection of bacterial DNA, which has promising prospects and potential applications in the quality control of food and water.
Year
DOI
Venue
2019
10.3390/s19245473
SENSORS
Keywords
DocType
Volume
DNA biosensor,ZnO nanorod arrays,LAPS,label-free detection,E. coli
Journal
19
Issue
ISSN
Citations 
24
1424-8220
0
PageRank 
References 
Authors
0.34
0
8
Name
Order
Citations
PageRank
Yulan Tian100.34
Tao Liang200.34
Ping Zhu300.34
Yating Chen400.34
Wei Chen500.34
Liping Du624.45
Chunsheng Wu700.68
Ping Wang81012.69