Paper Info
Title
LEMON: a method to construct the local strains at horizontal gene transfer sites in gut metagenomics.
Abstract
Horizontal Gene Transfer (HGT) refers to the transfer of genetic materials between organisms through mechanisms other than parent-offspring inheritance. HGTs may affect human health through a large number of microorganisms, especially the gut microbiomes which the human body harbors. The transferred segments may lead to complicated local genome structural variations. Details of the local genome structure can elucidate the effects of the HGTs. In this work, we propose a graph-based method to reconstruct the local strains from the gut metagenomics data at the HGT sites. The method is implemented in a package named LEMON. The simulated results indicate that the method can identify transferred segments accurately on reference sequences of the microbiome. Simulation results illustrate that LEMON could recover local strains with complicated structure variation. Furthermore, the gene fusion points detected in real data near HGT breakpoints validate the accuracy of LEMON. Some strains reconstructed by LEMON have a replication time profile with lower standard error, which demonstrates HGT events recovered by LEMON is reliable. Through LEMON we could reconstruct the sequence structure of bacteria, which harbors HGT events. This helps us to study gene flow among different microbial species.
Year
DOI
Venue
2019
10.1186/s12859-019-3301-8
BMC Bioinformatics
Keywords
Field
DocType
HGT, Local strain, Gut metagenomics, Graph
Genome,Graph,Biology,Horizontal gene transfer,Microbiome,Metagenomics,Gene flow,Computational biology,Genetics,DNA microarray,Human health
Journal
Volume
Issue
ISSN
23
suppl
1471-2105
Citations 
PageRank 
References 
0
0.34
0
Authors
3
Name
Order
Citations
PageRank
Chen Li100.34
Yiqi Jiang201.01
Shuai Cheng Li318430.25