Title
Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite.
Abstract
The determination of antibiotics in food is important due to their negative effect on human health related to antimicrobial resistance problem, renal toxicity, and allergic effects. We propose an impedimetric aptasensor for the determination of kanamycin A (KANA), which was assembled on the glassy carbon electrode by the deposition of carbon black in a chitosan matrix followed by carbodiimide binding of aminated aptamer mixed with oligolactide derivative of thiacalix[4]arene in aconeconfiguration. The assembling was monitored by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. In the presence of the KANA, the charge transfer resistance of the inner interface surprisingly decreased with the analyte concentration within 0.7 and 50 nM (limit of detection 0.3 nM). This was attributed to the partial shielding of the negative charge of the aptamer and of its support, a highly porous 3D structure of the surface layer caused by a macrocyclic core of the carrier. The use of electrostatic assembling in the presence of cationic polyelectrolyte decreased tenfold the detectable concentration of KANA. The aptasensor was successfully tested in the determination of KANA in spiked milk and yogurt with recoveries within 95% and 115%.
Year
DOI
Venue
2020
10.3390/s20174738
SENSORS
Keywords
DocType
Volume
electrochemical biosensor,electrochemical impedance spectroscopy,kanamycin,thiacalix[4]arene,milk contamination
Journal
20
Issue
ISSN
Citations 
17
1424-8220
0
PageRank 
References 
Authors
0.34
0
6
Name
Order
Citations
PageRank
Tatiana Kulikova100.34
Vladimir Gorbatchuk200.34
Ivan Stoikov300.34
Alexey Rogov400.34
Gennady Evtugyn522.56
Tibor Hianik603.04