Paper Info
Title
Acrdb: A Database Of Anti-Crispr Operons In Prokaryotes And Viruses
Abstract
CRISPR-Cas is an anti-viral mechanism of prokaryotes that has been widely adopted for genome editing. To make CRISPR-Cas genome editing more controllable and safer to use, anti-CRISPR proteins have been recently exploited to prevent excessive/prolonged Cas nuclease cleavage. Anti-CRISPR (Acr) proteins are encoded by (pro)phages/(pro)viruses, and have the ability to inhibit their host's CRISPR-Cas systems. We have built an online database AcrDB (http://bcb.unl.edu/AcrDB) by scanning similar to 19 000 genomes of prokaryotes and viruses with AcrFinder, a recently developed Acr-Aca (Acr-associated regulator) operon prediction program. Proteins in Acr-Aca operons were further processed by two machine learning-based programs (AcRanker and PaCRISPR) to obtain numerical scores/ranks. Compared to other anti-CRISPR databases, AcrDB has the following unique features: (i) It is a genome-scale database with the largest collection of data (39 799 Acr-Aca operons containing Aca or Acr homologs); (ii) It offers a user-friendly web interface with various functions for browsing, graphically viewing, searching, and batch downloading Acr-Aca operons; (iii) It focuses on the genomic context of Acr and Aca candidates instead of individual Acr protein family and (iv) It collects data with three independent programs each having a unique data mining algorithm for cross validation. AcrDB will be a valuable resource to the anti-CRISPR research community.
Year
DOI
Venue
2021
10.1093/nar/gkaa857
NUCLEIC ACIDS RESEARCH
DocType
Volume
Issue
Journal
49
D1
ISSN
Citations 
PageRank 
0305-1048
0
0.34
References 
Authors
0
9
Name
Order
Citations
PageRank
Le Huang121.73
Bowen Yang200.68
Haidong Yi333.17
Amina Asif455.51
jiawei wang53711.22
Trevor Lithgow6153.16
Han Zhang700.68
Fayyaz ul Amir Afsar Minhas8279.37
Yanbin Yin9317.75