Title
Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods.
Abstract
This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of beta-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.
Year
DOI
Venue
2020
10.3390/s20195594
SENSORS
Keywords
DocType
Volume
plasmin,trypsin,protease,casein,cleavage,acoustic sensor,thickness shear mode,quartz crystal microbalance,high-resolution ultrasonic spectroscopy
Journal
20
Issue
ISSN
Citations 
19
1424-8220
0
PageRank 
References 
Authors
0.34
0
3
Name
Order
Citations
PageRank
Mark Dizon100.34
Marek Tatarko200.34
Tibor Hianik303.04