Paper Info
Title
AC Electrothermal Flow-Enhanced, Label-Free Immunosensor for Rapid Electrochemical Sensing
Abstract
We report a label-free immunosensor employing AC electrothermal flow- (ACEF-) induced mixing for rapid electrochemical measurements of protein biomarkers. Compared with conventional immunoassays that require long (> 1 hr) incubation periods, ACEF-induced mixing significantly reduces the time required for immunocomplex formation while also enhancing the detection sensitivity. Numerical simulations and experimental studies were performed to validate the ACEF mixing approach, and optimize the ACEF operating parameters. Through cyclic voltammetry and fluorescence imaging, we show that ACEF mixing enhances antigen-antibody binding resulting in improved detection sensitivity compared with incubation- based immunocomplex formation. For proof of concept, this immunosensor was used for quantitative measurements of Plasmodium falciparum histidine-rich protein 2 (PfHRP2), a biomarker for malaria infection, in buffer samples, which could be detected from 1 ng/mL - 10,000 ng/mL in <; 10 min with excellent specificity.
Year
DOI
Venue
2020
10.1109/NEMS50311.2020.9265631
2020 IEEE 15th International Conference on Nano/Micro Engineered and Molecular System (NEMS)
Keywords
DocType
ISSN
label-free immunosensor,rapid electrochemical measurements,protein biomarkers,ACEF-induced mixing,immunocomplex formation,cyclic voltammetry,fluorescence imaging,antigen-antibody binding,rapid electrochemical sensing,AC electrothermal flow-enhanced immunosensor,incubation- ased immunocomplex formation,Plasmodium falciparum histidine-rich protein 2,malaria infection,biomarker
Conference
2474-3747
ISBN
Citations 
PageRank 
978-1-7281-7231-6
0
0.34
References 
Authors
0
2
Name
Order
Citations
PageRank
Jiran Li100.34
Peter B. Lillehoj200.34