Paper Info
Title
T4 Bacteriophage based Extended Gate Field Effect Transistors (T4B-EGFETs) for Bacteria Detection
Abstract
A T4 Bacteriophage based Extended Gate Field Effect Transistor (T4B-EGFET) was developed for viable bacteria detection. T4 bacteriophages were chemically immobilized on the surface of the extended gate connected to a MOSFET device for sensitive and specific detection of E. coli B bacteria cells. The capturing of the target bacteria cells by the anchored phages will induce a chemical potential, resulting in the shift of the threshold voltage, based on which the limit of detection (LOD) was obtained as 14±3 cfu/mL with a wide dynamic detection range (10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">~</sub> 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">8</sup> cfu/mL). Generalized g <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> /I <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">d</sub> theory was employed for normalized electronic-electrochemical sensitivity analysis and the FET optimized working regime had been identified to be moderate inversion for bacteria detection.
Year
DOI
Venue
2020
10.1109/NEMS50311.2020.9265586
2020 IEEE 15th International Conference on Nano/Micro Engineered and Molecular System (NEMS)
Keywords
DocType
ISSN
T4 bacteriophage,extended gate field effect transistors,T4B-EGFET,MOSFET device,E. coli B bacteria cell detection,chemical potential,electronic-electrochemical sensitivity analysis
Conference
2474-3747
ISBN
Citations 
PageRank 
978-1-7281-7231-6
0
0.34
References 
Authors
1
2
Name
Order
Citations
PageRank
Jingting Xu100.34
Yi-Kuen Lee2711.16