Name
Abstract | ||
|---|---|---|
Antiviral therapies targeting the pandemic coronavirus disease 2019 (COVID-19) are urgently required. We studied an already-approved botanical drug cepharanthine (CEP) in a cell culture model of GX_P2V, a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-related virus. RNA-sequencing results showed the virus perturbed the expression of multiple genes including those associated with cellular stress responses such as endoplasmic reticulum (ER) stress and heat shock factor 1 (HSF1)-mediated heat shock response, of which heat shock response-related genes and pathways were at the core. CEP was potent to reverse most dysregulated genes and pathways in infected cells including ER stress/unfolded protein response and HSF1-mediated heat shock response. Additionally, single-cell transcriptomes also confirmed that genes of cellular stress responses and autophagy pathways were enriched in several peripheral blood mononuclear cells populations from COVID-19 patients. In summary, this study uncovered the transcriptome of a SARS-CoV-2-related coronavirus infection model and anti-viral activities of CEP, providing evidence for CEP as a promising therapeutic option for SARS-CoV-2 infection. |
Year | DOI | Venue |
|---|---|---|
2021 | 10.1093/bib/bbaa387 | BRIEFINGS IN BIOINFORMATICS |
Keywords | DocType | Volume |
SARS-CoV-2, COVID-19, GX_P2V, cellular stress, cepharanthine, transcriptome | Journal | 22 |
Issue | ISSN | Citations |
2 | 1467-5463 | 0 |
PageRank | References | Authors |
0.34 | 0 | 10 |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Shasha Li | 1 | 0 | 0.34 |
| Wenli Liu | 2 | 0 | 0.34 |
| Yangzhen Chen | 3 | 0 | 0.34 |
| Liqin Wang | 4 | 0 | 0.34 |
| Wenlin An | 5 | 0 | 0.34 |
| Xiaoping An | 6 | 0 | 0.34 |
| Lihua Song | 7 | 0 | 0.34 |
| Yigang Tong | 8 | 1 | 2.40 |
| Huahao Fan | 9 | 0 | 1.01 |
| Chenyang Lu | 10 | 0 | 0.34 |