Name
Title | ||
|---|---|---|
Evaluation Of Antidiabetic Activity Of Dietary Phenolic Compound Chlorogenic Acid In Streptozotocin Induced Diabetic Rats: Molecular Docking, Molecular Dynamics, In Silico Toxicity, In Vitro And In Vivo Studies |
Abstract | ||
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Background: Chlorogenic acid is amongst the well-known polyphenolic compounds being used in human food and beverages. Its presence has been reported in tea leaves, roasted green beans, coffee, cocoa, berry fruits, apples, citrus fruits, and pears.Objective: The present study aims to elucidate the effectiveness of chlorogenic acid on in silico and in vitro inhibition of glucose metabolising enzymes (alpha-amylase and alpha-glucosidase) and on blood-based markers associated with diabetic complications in vivo.Methods: Docking and molecular dynamics studies were performed using GLIDE (Schrodinger, LLC, NY, 2019-2) and Maestro-Desmond Interoperability Tools, version 4.1 (Schrodinger, NY, 2015), respectively. alpha-Amylase and alpha-glucosidase inhibitory activities of chlorogenic acid were measured in vitro. Diabetes was induced in adult Wistar rats by injecting streptozotocin (50 mg/kg). Biochemical assays were performed using standard kits.Result: The in silico studies for alpha-amylase and alpha-glucosidase with chlorogenic acid suggested that the ligand was stable and strongly bound with the above-mentioned proteins. During in vitro studies, chlorogenic acid inhibited both the enzymes in a dose-dependent manner (5-30 mu g/mL). In addition, chlorogenic acid treatment for 28 days significantly suppressed the increase in blood glucose, total cholesterol, triglyceride, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, gamma-glutamyl transferase, alkaline phosphatase, total bilirubin, creatinine, urea, uric acid, and feed intake levels in diabetic rats. Chlorogenic acid also caused significant improvement in body weight, serum HDL-cholesterol, total protein, and albumin levels leading to betterment in atherogenic indices related to diabetes-associated cardiovascular risks.Conclusion: The findings indicated that chlorogenic acid inhibited alpha-amylase and alpha-glucosidase and significantly decreased diabetes associated hyperglycemia, hyperlipidemia, and hepatorenal damage, making it a possible functional food ingredient and drug candidate for the management of diabetes and related complications. |
Year | DOI | Venue |
|---|---|---|
2021 | 10.1016/j.compbiomed.2021.104462 | COMPUTERS IN BIOLOGY AND MEDICINE |
Keywords | DocType | Volume |
Diabetes, ADMET, alpha-amylase, alpha-glucosidase, Streptozotocin, Chlorogenic acid | Journal | 134 |
ISSN | Citations | PageRank |
0010-4825 | 0 | 0.34 |
References | Authors | |
0 | 6 | |
Name | Order | Citations | PageRank |
|---|---|---|---|
| Amit Kumar Singh | 1 | 0 | 0.34 |
| Harvesh Kumar Rana | 2 | 0 | 0.34 |
| Vishal Veer Singh | 3 | 6 | 3.95 |
| Tara Chand Yadav | 4 | 0 | 0.34 |
| Pritish Varadwaj | 5 | 0 | 0.34 |
| Abhay Kumar Pandey | 6 | 0 | 0.34 |