Title
Video-Rate Quantitative Phase Imaging Using a Digital Holographic Microscope and a Generative Adversarial Network
Abstract
The conventional reconstruction method of off-axis digital holographic microscopy (DHM) relies on computational processing that involves spatial filtering of the sample spectrum and tilt compensation between the interfering waves to accurately reconstruct the phase of a biological sample. Additional computational procedures such as numerical focusing may be needed to reconstruct free-of-distortion quantitative phase images based on the optical configuration of the DHM system. Regardless of the implementation, any DHM computational processing leads to long processing times, hampering the use of DHM for video-rate renderings of dynamic biological processes. In this study, we report on a conditional generative adversarial network (cGAN) for robust and fast quantitative phase imaging in DHM. The reconstructed phase images provided by the GAN model present stable background levels, enhancing the visualization of the specimens for different experimental conditions in which the conventional approach often fails. The proposed learning-based method was trained and validated using human red blood cells recorded on an off-axis Mach-Zehnder DHM system. After proper training, the proposed GAN yields a computationally efficient method, reconstructing DHM images seven times faster than conventional computational approaches.
Year
DOI
Venue
2021
10.3390/s21238021
SENSORS
Keywords
DocType
Volume
phase compensation, digital holographic microscopy, learning-based method, generative adversarial networks, video-rate performance
Journal
21
Issue
ISSN
Citations 
23
1424-8220
0
PageRank 
References 
Authors
0.34
0
3
Name
Order
Citations
PageRank
Raul Castaneda100.34
Carlos Trujillo200.34
Ana Doblas302.03